An E. coli strain that carries the NaeI gene from Nocardia aerocolonigenes (ATCC 23870).
Reagents Supplied
The following reagents are supplied with this product:
Store at (°C)
Concentration
CutSmart® Buffer
-20
10X
Notes:
Demonstrates marked site preferences and cuts pBR322 DNA very slowly.Inhibited by salt concentrations > 50mM.NgoMIV, an isoschizomer of NaeI, exhibits less site preference.For enzymes that cannot be heat-inactivated, we recommend using a column for cleanup (such as the Monarch? PCR & DNA Cleanup Kit), or running the reaction on an agarose gel and then extracting the DNA (we recommend Monarch Gel Extraction Kit), or performing a phenol/chloroform extraction.