High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design.NEB extensively performs quality controls on all standard and high-fidelity(HF) restriction enzymes. Examples of nuclease contamination studies for someof our HF restriction enzymes are shown below.
Product Source
AnE. colistrain that carries the cloned and modified HindIII gene fromHaemophi-lus influenzae Rd (ATCC 51907)
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