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Abnova/SARS-CoV-2 N recombinant monoclonal antibody, clone 85C10 (Biotin)/1kit/RAB01062
  • Abnova/SARS-CoV-2 N recombinant monoclonal antibody, clone 85C10 (Biotin)/1kit/RAB01062

Abnova/SARS-CoV-2 N recombinant monoclonal antibody, clone 85C10 (Biotin)/1kit/RAB01062

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貨號(hào): RAB01062
品牌: Abnova
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      • Specification
      • Specification
      • Product Description:
      • Recombinant rabbit monoclonal antibody raised against SARS-CoV-2 N.
      • Immunogen:
      • A synthetic peptide corresponding to C-terminus of SARS-CoV-2 N.
      • Host:
      • Rabbit
      • Reactivity:
      • SARS-CoV-2
      • Specificity:
      • Highly-specific to SARS-CoV-2 nucleoprotein; does not cross-react with nucleoprotein from SARS-CoV or other coronaviruses.
      • Form:
      • Liquid
      • Conjugation:
      • Biotin
      • Preparation Method:
      • 85C10 is derived from a Fab phage display library made from a rabbit immunized with synthetic peptide specific to the C-terminus of SARS-CoV-2 nucleocapsid protein. Libraries were selected on N (SARS-CoV-2) Recombinant Protein (Cat # P6687) and Fab sup were screened on SARS-CoV-2 NP and SARS-CoV (2003) NP by ELISA. Positive clones were cloned into a bi-cistronic IgG vector and produced in HEK293 cells. The antibodies were purified from a Protein A column.
      • Purification:
      • Protein A purification
      • Concentration:
      • 0.5 mg/mL
      • Isotype:
      • IgG1, kappa
      • Recommend Usage:
      • ELISAThe optimal working dilution should be determined by the end user.
      • Storage Buffer:
      • In 1X PBS (0.02% sodium azide).
      • Storage Instruction:
      • Store at 4°C.
      • Note:
      • For highest sensitivity, we recommend pairing unconjugated clone 75G5a (Cat # RAB01053), immobilized on the ELISA plate to capture COVID-19 NP antigen, with SARS-CoV-2 N recombinant monoclonal antibody, clone 85C10 (Biotin) (Cat # RAB01062) for detection using high-sensitivity streptavidin-HRP (Pierce # 21130). Other unconjugated and biotinylated anti-NP clones are provided as alternatives and also show high sensitivity and specificity. 75G5a (Cat # RAB01053), 84C4a (Cat # RAB01055), 84D7 (Cat # RAB01057), 85C1 (Cat # RAB01059), 85C10 (Cat # RAB01061), 85B4 (Cat # RAB01063), and 85E9 (Cat # RAB01065) can be directly coated to the ELISA wells as a capture antibody and the other antibody (e.g. biotinylated) [75G5a (Cat # RAB01054), 84C4a (Cat # RAB01056), 84D7 (Cat # RAB01058), 85C1 (Cat # RAB01060), 85C10 (Cat # RAB01062), 85B4 (Cat # RAB01064), and 85E9 (Cat # RAB01066)] can be used as a detecting antibody with StreptAvidin-HRP.This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
      • Datasheet:
      • PDF DownloadDownload
      • Applications
      • Enzyme-linked Immunoabsorbent Assay
      • Enzyme-linked Immunoabsorbent Assay
      • Microtiter wells were coated with N (SARS-CoV-2) Recombinant Protein (Cat # P6687) at 1 ug/mL. SARS-CoV-2 N recombinant monoclonal antibody, clone 85C10 (Biotin) (Cat # RAB01062) was serially diluted 1:2 starting at 1 ug/mL, and shows high-sensitivity binding to COVID-19 NP antigen.
      • Sandwich ELISA
      • Sandwich ELISA
      • A sandwich ELISA was performed using SARS-CoV-2 N recombinant monoclonal antibody, clone 75G5a (Cat # RAB01053) as a capture antibody and SARS-CoV-2 N recombinant monoclonal antibody, clone 85C10 (Biotin) (Cat # RAB01062) as a detection antibody. COVID-19 NP was serially diluted 1:2 starting at 20 ng/mL. Recombinant rabbit monoclonal antibodies 75G5a and 85C10-biotin detected COVID-19 NP antigen at very high sensitivity as low as 19.5 pg/mL (1.9 pg).
      • Application Image
      • Enzyme-linked Immunoabsorbent Assay
      • Enzyme-linked Immunoabsorbent Assay
      • enlarge
      • Sandwich ELISA
      • Sandwich ELISA
      • enlarge
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