產(chǎn)品概述：儲(chǔ)存條件4℃密封避光保存，短期可放置于室溫。有效期見(jiàn)外包裝。
組分 規(guī)格 | S6009S（10mL） | S6009M（100mL） | S6009L（500mL） |
A液 | 5 mL | 50 mL | 250 mL |
B液 | 5 mL | 50 mL | 250 mL |
產(chǎn)品介紹Super ECL Plus 免疫印跡底物是增強(qiáng)型化學(xué)發(fā)光（ECL）過(guò)氧化物酶的底物，可幫助用戶(hù)在免疫印跡分析過(guò)程中實(shí)現(xiàn)低表達(dá)或高價(jià)值蛋白的檢測(cè)。Super ECL Plus 底物可為使用辣根過(guò)氧化物酶（HRP）偶聯(lián)物的免疫印跡實(shí)驗(yàn)提供明亮的信號(hào)，對(duì)低表達(dá)或高價(jià)值蛋白檢測(cè)靈敏度很高。該 ECL 底物能夠兼容各種膜、封閉液和寬范圍抗體稀釋液，以出色性能、通用性和高性?xún)r(jià)比，滿(mǎn)足用戶(hù)的免疫印跡應(yīng)用需求。Super ECL Plus（超敏）特點(diǎn)：1. 具有極高靈敏度：檢測(cè)硝化纖維素膜或 PVDF 膜上低表達(dá)或高價(jià)值的蛋白條帶；2. 長(zhǎng)信號(hào)持續(xù)時(shí)間：條件優(yōu)化的情況下，經(jīng)底物孵育的印跡條帶能夠持續(xù)輸出 6 至 8 h 的可檢測(cè)光信號(hào)；3. 價(jià)格經(jīng)濟(jì)：配方經(jīng)過(guò)優(yōu)化，適用于濃度極低的抗體檢測(cè)。? 0.2-1 μg/mL 一抗（或 1 mg/mL 的稀釋 1:1,000-1:5,000）? 10-50 ng/mL 二抗（或 1 mg/mL 的稀釋 1:20,000-1:100,000）注意事項(xiàng)1. 發(fā)光液曝露于強(qiáng)光下時(shí)間過(guò)久靈敏度可能有降低，操作時(shí)注意避光。戴手套可以避免在膜上留下手印。2. 長(zhǎng)時(shí)間曝光會(huì)加深背景；蛋白過(guò)量，會(huì)使條帶強(qiáng)弱變化失去線(xiàn)性關(guān)系；曝光不足，則條帶模糊或較淺。3. 如果曝光后條帶不佳，可用洗膜緩沖液洗膜，重新孵育二抗，然后重新用 ECL 曝光。4. 使用肉眼可見(jiàn)的預(yù)染色蛋白 Marker 和熒光-放射自顯影曝光標(biāo)簽可精確確定膠片上條帶的位置和大小。5. NaN3 會(huì)抑制 HRP 活性，回收二抗應(yīng)避免使用 NaN3，如必需使用勿超過(guò) 0.01%。
使用本產(chǎn)品的文獻(xiàn)：1.CtIP promotes G2/M arrest in etoposide‐treated HCT116cells in a p53‐independent mannerHongyu Chen，Jin Shan，Dandan Chen，Ruoxi Wang，Wenjing Qi，Hailong Wang，YUEshuang Ke，Wenguang Liu，Xianlu ZengJournal of cellular physiology(2018)2.MeningiticEscherichia coli-induced upregulation of PDGF-B and ICAM-1 aggravates blood-brain barrier disruption and neuroinflammatory responseRui-Cheng Yang,Xin-Yi Qu,Si-Yu Xiao,Liang Li,Bo-Jie Xu,Ji-Yang Fu,Yu-Jin Lv,Nouman Amjad,Chen Tan,Kwang Sik Kim,Huan-Chun Chen andXiang-Ru WangJournal of Neuroinflammation（2019 16：101） 3.Melatonin enhances thrombopoiesis through ERK and Akt activation orchestrated by dual adaptor for phosphotyrosine and 3‐phosphoinositides Shilei Chen，Yan Qi，Song Wang，Yang Xu，Mingqiang Shen，Mengjia Hu，Changhong Du，F(xiàn)ang Chen，Mo Chen，Yukai Lu，Zihao Zhang，Yong Quan，Cheng Wang，F(xiàn)engchao Wang，Junping Wang J Pineal Res 4.LncRNAs activate longevity regulation pathway due to aging of Leydig cellscaused by DEHP exposure: A transcriptome-based study Yuhao Wu，Junke Wang，Tianxin Zhao，Yuexin Wei，Lindong Han，Lianju Shen，Chunlan Long，Shengde Wu，Guanghui Wei Ecotoxicology and Environmental Safety5.Propionate and butyrate produced by gut microbiota after probiotic supplementation attenuate lung metastasis of melanoma cells in mice Lili Chen,Xinyu Zhou,Yawei Wang,Dake Wang,Yueshuang Ke,Xianlu Zeng Molecular Nutrition & Food Research 6.Orientin mediates protection against MRSA-induced pneumonia by inhibiting Sortase ALi Wang,Shisong Jing,Han Qu,Kai Wang,Yajing Jin,Ying Ding,Lin Yang,Hangqian Yu,Yan Shi,Qianxue Li,Dacheng WangVirlence Volime 127.Single-Base Resolution Mapping Reveals Distinct 5-Formylcytidine in Saccharomyces cerevisiae mRNAsYafen Wang,Zonggui Chen,Xiong Zhang,Xiaocheng Weng,Jikai Deng,Wei Yang,Fan Wu,Shaoqing Han,Chao Xia,Yu Zhou,Yu Chen,Xiang ZhouACS Chemical Biology
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UE-S6009S/S6009M/S6009L
