This package contains 50 nmol of SNAP-Biotin substrate, sufficient to make 10 ml of a 5 μM solution for the labeling of SNAP-tag fusion proteins in cells.The SNAP-tag protein labeling system enables the specific, covalent attachment of virtually any molecule to a protein of interest. The SNAP-tag is based on mammalian O6-alkylguanine-DNA-alkyltransferase (AGT). SNAP-tag substrates are derivatives of benzylpurines and benzylchloropyrimidines, In the labeling reaction, the substituted benzyl group of the substrate becomes covalently attached to the SNAP-tag.There are two steps to using this system: subcloning and expression of the protein of interest as a SNAP-tag fusion, and labeling of the fusion with the SNAP-tag substrate of choice. Expression of SNAP-tag fusion proteins is described in the documentation supplied with SNAP-tag plasmids. The labeling of fusion proteins with the SNAP-Biotin substrate is described below.Figure 1:Structure of SNAP-Biotin (MW 609.7 g/mol)
This product is related to the following categories:
Biotin Labels Products,
SNAP-tag Substrates Products ,
Protein Labeling Products
This product can be used in the following applications:
購物車為空，快去購物吧！
4000-520-616
咨詢顧問
特別 提示
