Synonym:TriglycerideandFreeGlycerolKitsandReagentsProperties:
RelatedCategories | BiochemicalsandReagents,BloodandUrineChemistryAssayKits,CellBIOLOGy,EnzymaticKitsfortheQuantitationofNutrientsandMetabolites,FattyAcid&LipidMetabolismAssayKits, |
usage | 1 kitsufficientfor250 tests |
storagetemp. | 2-8°C |
Description:
FrequentlyAskedQuestions
FrequentlyAskedQuestionsareavailableforthisProduct.
Linkage
Inadditiontokits,theindividualreagentsandglycerolstandardareavailableseparatelywhenfewerreactionsareneeded.
Packaging
Thekitalsoincludessufficientreagentforanadditional250freeglyceridetestsfortruetriglyceridedetermination.
Application
ASerumTriglycerideDeterminationKitwasusedtodeterminetheleveloftriglyceridesinwhole-bodyhomogenatesofDrosophilamelanogaster.
TheSerumTriglycerideDeterminationKitcanbeusedforthemeasurementofglycerol,truetriglycerides,ortotaltriglyceridesinserumorplasma.Theprocedureinvolvesenzymatichydrolysisbylipaseofthetriglyceridestoglycerolandfreefattyacids.Theglycerolproducedisthenmeasuredbycoupledenzymereactions.Manyofthetriglyceridereagentswhicharecommerciallyavailable,donotdifferentiatebetweenendogenousglycerolandglycerolderivedbyhydrolyticactionoflipaseonglycerides.
Triglyceridesarefirsthydrolyzedbylipoproteinlipasetoglycerolandfreefattyacids.Glycerolisthenphosphorylatedbyadenosine-5′-triphosphate(ATP)formingglycerol-1-phosphate(G-1-P)andadenosine-5′-diphosphate(ADP)inthereactioncatalyzedbyglycerolkinase(GK).G-1-Pisthenoxidizedbyglycerolphosphateoxidase(GPO)todihydroxy-acetonephosphate(DAP)andhydrogenperoxide(H2O2).Peroxidase(POD)catalyzesthecouplingofH2O2with4-aminoantipyrine(4-AAP)andsodium
N-ethyl-N-(3-sulfopropyl)m-anisidine(ESPA)toproduceaquinoneiminedyethatshowsanabsorbancemaximumat540nm.Theincreaseinabsorbanceat540nmisdirectlyproportionaltotriglycerideconcentrationofthesample.
Thetriglycerideandfreeglycerolkitsareforthequantitativedeterminationofglycerol,totaltriglyceridesorfreetriglycerides.
Safety:
Symbol
GHS07,GHS09
Hazardstatements
H319-H411
Precautionarystatements
P273-P280-P305+P351+P338-P337+P313-P391-P501
RIDADR
NONHforallmodesoftransport